Study Preview
Study Title and Description
Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.
Key Questions Addressed
1 | Key Question 1 - Summary of matched comparisons of select assays for C. difficile toxins |
Primary Publication Information
Title | Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study. |
Author | van den Berg RJ., Vaessen N., Endtz HP., Schülin T., van der Vorm ER., Kuijper EJ. |
Country | Department of Medical Microbiology, E4-67, Centre of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands. |
Year | 2007 |
Numbers |
Pubmed ID: 17172514 |
Secondary Publication Information
There are currently no secondary publications defined for this study.
Extraction Form: Key Question 1 - Summary of matched comparisons of select assays for C. difficult toxins (Table C1)
Question... Follow Up | Answer | Follow-up Answer | |
---|---|---|---|
Patients/ Site | n=450 all with diarrhea, some suspected CDI others not but inpatients for at least 72 hours; from 4 medical centers in The Netherlands; Unknown spectrum Unknown selection | ||
Tested Specimens | n=547 diarrhea frozen at - 20o C; Unknown blinded | ||
Reference Standard/ % Positive in Sample | CTA 5.7% | ||
Tests Compared | Premier Toxins A&B, Meridian VIDAS C. difficile Tox A II, bioMerieux Vitek | ||
PPV/ NPV | Premier Toxins A&B, Meridian PPV 30/59=50.8% NPV 480/481=99.8% VIDAS C. difficile Tox A II, bioMerieux Vitek PPV 26/41=63.4% NPV 494/499=99.0% | ||
True Positive/ False Positive‡ | Premier Toxins A&B, Meridian True Positive 30/31=96.8% False Positive 29/509=5.7% VIDAS C. difficile Tox A II, bioMerieux Vitek True Positive 26/31=83.8% False Positive 15/509=2.9% |
Results & Comparisons
No Results found.