Study Title and Description
Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.
Key Questions Addressed
|1||Key Question 1 - Summary of matched comparisons of select assays for C. difficile toxins|
Primary Publication Information
|Title||Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.|
|Author||van den Berg RJ., Vaessen N., Endtz HP., Schülin T., van der Vorm ER., Kuijper EJ.|
|Country||Department of Medical Microbiology, E4-67, Centre of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands.|
Pubmed ID: 17172514
Secondary Publication Information
There are currently no secondary publications defined for this study.
Extraction Form: Key Question 1 - Summary of matched comparisons of select assays for C. difficult toxins (Table C1)
|Question... Follow Up||Answer||Follow-up Answer|
|Patients/ Site||n=450 all with diarrhea, some suspected CDI others not but inpatients for at least 72 hours; from 4 medical centers in The Netherlands; Unknown spectrum Unknown selection|
|Tested Specimens||n=547 diarrhea frozen at - 20o C; Unknown blinded|
|Reference Standard/ % Positive in Sample||CTA 5.7%|
|Tests Compared||Premier Toxins A&B, Meridian VIDAS C. difficile Tox A II, bioMerieux Vitek|
|PPV/ NPV||Premier Toxins A&B, Meridian PPV 30/59=50.8% NPV 480/481=99.8% VIDAS C. difficile Tox A II, bioMerieux Vitek PPV 26/41=63.4% NPV 494/499=99.0%|
|True Positive/ False Positive‡||Premier Toxins A&B, Meridian True Positive 30/31=96.8% False Positive 29/509=5.7% VIDAS C. difficile Tox A II, bioMerieux Vitek True Positive 26/31=83.8% False Positive 15/509=2.9%|
Results & Comparisons
No Results found.