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Study Title and Description

Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.



Key Questions Addressed
1 Key Question 1 - Summary of matched comparisons of select assays for C. difficile toxins
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Primary Publication Information
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TitleData
Title Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.
Author van den Berg RJ., Vaessen N., Endtz HP., Schülin T., van der Vorm ER., Kuijper EJ.
Country Department of Medical Microbiology, E4-67, Centre of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands.
Year 2007
Numbers Pubmed ID: 17172514

Secondary Publication Information
There are currently no secondary publications defined for this study.


Extraction Form: Key Question 1 - Summary of matched comparisons of select assays for C. difficult toxins (Table C1)
Design Details
Question... Follow Up Answer Follow-up Answer
Patients/ Site n=450 all with diarrhea, some suspected CDI others not but inpatients for at least 72 hours; from 4 medical centers in The Netherlands; Unknown spectrum Unknown selection
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Tested Specimens n=547 diarrhea frozen at - 20o C; Unknown blinded
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Reference Standard/ % Positive in Sample CTA 5.7%
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Tests Compared Premier Toxins A&B, Meridian VIDAS C. difficile Tox A II, bioMerieux Vitek
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PPV/ NPV Premier Toxins A&B, Meridian PPV 30/59=50.8% NPV 480/481=99.8% VIDAS C. difficile Tox A II, bioMerieux Vitek PPV 26/41=63.4% NPV 494/499=99.0%
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True Positive/ False Positive‡ Premier Toxins A&B, Meridian True Positive 30/31=96.8% False Positive 29/509=5.7% VIDAS C. difficile Tox A II, bioMerieux Vitek True Positive 26/31=83.8% False Positive 15/509=2.9%
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Results & Comparisons

No Results found.